Usually: no.When WordPress is running on apache with mod_rewrite enabled, it'll use
# BEGIN WordPress<IfModule mod_rewrite.c>RewriteEngine OnRewriteBase /RewriteRule ^index\.php$ - [L]RewriteCond %{REQUEST_FILENAME} !-fRewriteCond %{REQUEST_FILENAME} !-dRewriteRule . /index.php [L]</IfModule># END WordPressTo make sure that static files will NOT be served through WP.A plugin might add its own rewrite rules to the .htaccess, but generally WP will not interfere with static files, those will be dealt with by apache directly.